Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Hepcidin induces HIV-1 transcription inhibited by ferroportin. | RETROVIROLOGY Hepcidin induces HIV-1 transcription inhibited by ferroportin Xu et al. Xu et al. Retrovirology 2010 7 104 http content 7 1 104 2 December 2010 BioMed Central Xu et al. Retrovirology 2010 7 104 http content 7 1 104 RESEARCH RETR0VIR0L0GY Open Access Hepcidin induces HIV-1 transcription inhibited by ferroportin 1 3 12 1 Min Xu Fatah Kashanchi Altreisha Foster Jamie Rotimi Willie Turner Victor R Gordeuk Sergei Nekhai Abstract Background Physiological regulation of cellular iron involves iron export by the membrane protein ferroportin the expression of which is induced by iron and negatively modulated by hepcidin. We previously showed that iron chelation is associated with decreased HIV-1 transcription. We hypothesized that increased iron export by ferroportin might be associated with decreased HIV-1 transcription and degradation of ferroportin by hepcidin might in turn induce HIV-1 transcription and replication. Here we analyzed the effect of ferroportin and hepcidin on HIV-1 transcription. Results Expression of ferroportin was associated with reduced HIV-1 transcription in 293T cells and addition of hepcidin to ferroportin-expressing cells counteracted this effect. Furthermore exposure of promonocytic THP-1 cells to hepcidin was associated with decreased ferroportin expression increased intracellular iron and induction of reporter luciferase gene expression. Finally exposure of human primary macrophages and CD4 T cells to hepcidin and iron was also associated with induction of viral production. Conclusion Our results suggest that the interplay between ferroportin-mediated iron export and hepcidin-mediated degradation of ferroportin might play a role in the regulation of HIV-1 transcription and may be important for understanding of HIV-1 pathogenesis. Background Movement of dietary iron from absorptive enterocytes to portal plasma and of macrophage iron to systemic plasma is mediated by the iron .
