Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Extensive complement-dependent enhancement of HIV-1 by autologous non-neutralising antibodies at early stages of infection. | Willey et al. Retrovirology 2011 8 16 http content 8 1 16 RETR0VIR0L0GY RESEARCH Open Access Extensive complement-dependent enhancement of HIV-1 by autologous non-neutralising antibodies at early stages of infection 1 3 3 3 1 Suzanne Willey 1 Marlén MI Aasa-Chapman Stephen O Farrell Pierre Pellegrino Ian Williams Robin A Weiss Stuart JD Neil1 2 Abstract Background Non-neutralising antibodies to the envelope glycoprotein are elicited during acute HIV-1 infection and are abundant throughout the course of disease progression. Although these antibodies appear to have negligible effects on HIV-1 infection when assayed in standard neutralisation assays they have the potential to exert either inhibitory or enhancing effects through interactions with complement and or Fc receptors. Here we report that non-neutralising antibodies produced early in response to HIV-1 infection can enhance viral infectivity. Results We investigated this complement-mediated antibody-dependent enhancement C -ADE of early HIV infection by carrying out longitudinal studies with primary viruses and autologous sera derived sequentially from recently infected individuals using a T cell line naturally expressing the complement receptor 2 CR2 CD21 . The C -ADE was consistently observed and in some cases achieved infection-enhancing levels of greater than 350-fold converting a low-level infection to a highly destructive one. C -ADE activity declined as a neutralising response to the early virus emerged but later virus isolates that had escaped the neutralising response demonstrated an increased capacity for enhanced infection by autologous antibodies. Moreover sera with autologous enhancing activity were capable of C ADE of heterologous viral isolates suggesting the targeting of conserved epitopes on the envelope glycoprotein. Ectopic expression of CR2 on cell lines expressing HIV-1 receptors was sufficient to render them sensitive to C ADE. Conclusions Taken together these .
