Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Latent Membrane Protein 1 as a molecular adjuvant for single-cycle lentiviral vaccines. | Gupta et al. Retrovirology 2011 8 39 http content 8 1 39 RETR0VIR0L0GY RESEARCH Open Access Latent Membrane Protein 1 as a molecular adjuvant for single-cycle lentiviral vaccines 1 1 1 2 Sachin Gupta James M Termini Liguo Niu Saravana K Kanagavelu Andrew R Rahmberg Richard S Kornbluth3 David T Evans2 and Geoffrey W Stone1 Abstract Background Molecular adjuvants are a promising method to enhance virus-specific immune responses and protect against HIV-1 infection. Immune activation by ligands for receptors such as CD40 can induce dendritic cell activation and maturation. Here we explore the incorporation of two CD40 mimics Epstein Barr Virus gene LMP1 or an LMP1-CD40 chimera into a strain of SIV that was engineered to be limited to a single cycle of infection. Results Full length LMP1 or the chimeric protein LMP1-CD40 was cloned into the nef-locus of single-cycle SIV. Human and Macaque monocyte derived macrophages and DC were infected with these viruses. Infected cells were analyzed for activation surface markers by flow cytometry. Cells were also analyzed for secretion of pro-inflammatory cytokines IL-1P IL-6 IL-8 IL-12p70 and TNF by cytometric bead array. Conclusions Overall single-cycle SIV expressing LMP1 and LMP1-CD40 produced a broad and potent TH1-biased immune response in human as well as rhesus macaque macrophages and DC when compared with control virus. Single-cycle SIV-LMP1 also enhanced antigen presentation by lentiviral vector vaccines suggesting that LMP1-mediated immune activation may enhance lentiviral vector vaccines against HIV-1. Background To develop an effective lentiviral vector vaccine against HIV-1 infection it may be necessary to focus on enhancing the activation of dendritic cells and other professional antigen presenting cells in order to maximize the stimulation of virus-specific immune responses. One of the critical events in the induction of immune response is the maturation of DCs and macrophages 1 . Maturing .
