Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall-derived mesenchymal stem cells. | Gibellini et al. Retrovirology 2011 8 40 http content 8 1 40 RETR0VIR0L0GY RESEARCH Open Access HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall-derived mesenchymal stem cells 1 t 2t 1 3 3 1 Davide Gibellini t Francesco Alviano t Anna Miserocchi Pier Luigi Tazzari Francesca Ricci Alberto Clò Ci i-s l lr rinỈ1 l l2 f r Rr ti Hori4 Diorli lini Í2lí24 212 212 H ro 2 D2C 2I liI2ol li5 D2 I 12 lo 2 2l 2 D2nli2r 23 Silvia Moiini Maico Boideii Pierluigi Viale Gianandiea Pasquinelli Pasqualepaolo Pagliaio Gian Paolo Bagnara2 and Maria Carla Re1 6 Abstract Background HIV infection elicits the onset of a progressive immunodeficiency and also damages several other organs and tissues such as the CNS kidney heart blood vessels adipose tissue and bone. In particular HIV infection has been related to an increased incidence of cardiovascular diseases and derangement in the structure of blood vessels in the absence of classical risk factors. The recent characterization of multipotent mesenchymal cells in the vascular wall involved in regulating cellular homeostasis suggests that these cells may be considered a target of HIV pathogenesis. This paper investigated the interaction between HIV-1 and vascular wall resident human mesenchymal stem cells MSCs . Results MSCs were challenged with classical R5 and X4 HIV-1 laboratory strains demonstrating that these strains are able to enter and integrate their retro-transcribed proviral DNA in the host cell genome. Subsequent experiments indicated that HIV-1 strains and recombinant gp120 elicited a reliable increase in apoptosis in sub-confluent MSCs. Since vascular wall MSCs are multipotent cells that may be differentiated towards several cell lineages we challenged HIV-1 strains and gp120 on MSCs differentiated to adipogenesis and endotheliogenesis. Our experiments showed that the adipogenesis is increased especially by upregulated PPARg activity whereas the endothelial .
