Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Interactions between human immunodeficiency virus (HIV)-1 Vpr expression and innate immunity influence neurovirulence. | Na et al. Retrovirology 2011 8 44 http content 8 1 44 RETR0VIR0L0GY RESEARCH Open Access Interactions between human immunodeficiency virus HIV -1 Vpr expression and innate immunity influence neurovirulence 1 14 1 2 Hong Na Shaona Acharjee Gareth Jones Pornpun Vivithanaporn 1 Farshid Noorbakhsh Nicola McFarlane corHỈn n 4 K ÌTỉindTỉi 1 141711 IC Rtỉ I Isỉnx i3 rlr iC A PíirHm6 Rri A nhoti7 Chrictrinhcir A or1 2 4 Ferdinand Maingai 1 Klaus Daiianyi 1 Cailos A Paido 1 lie A Conen and diiisiopnei Power Abstract Background Viral diversity and abundance are defining properties of human immunodeficiency virus HIV -1 s biology and pathogenicity. Despite the increasing availability of antiretroviral therapy HIV-associated dementia HAD continues to be a devastating consequence of HIV-1 infection of the brain although the underlying disease mechanisms remain uncertain. Herein molecular diversity within the HIV-1 non-structural gene Vpr was examined in RNA sequences derived from brain and blood of HIV AIDS patients with or without HIV-associated dementia HAD together with the ensuing pathobiological effects. Results Cloned brain- and blood-derived full length vpr alleles revealed that amino acid residue 77 within the brain-derived alleles distinguished HAD 77Q from non-demented ND HIV AIDS patients 77R p although vpr transcripts were more frequently detected in HAD brains p . Full length HIV-1 clones encoding the 77R-ND residue induced higher IFN-a MX1 and BST-2 transcript levels in human glia relative to the 77Q-HAD encoding virus p but both viruses exhibited similar levels of gene expression and replication. Myeloid cells transfected with 77Q- pVpr77Q-HAD 77R pVpr77R-ND or Vpr null pVprO-containing vectors showed that the pVpr77R-ND vector induced higher levels of immune gene expression p and increased neurotoxicity p . Vpr peptides amino acids 70-96 containing the 77Q-HAD or 77R-ND motifs induced similar levels of cytosolic
