Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: The HIV-1 Rev/RRE system is required for HIV-1 5’ UTR cis elements to augment encapsidation of heterologous RNA into HIV-1 viral particles. | Cockrell et al. Retrovirology 2011 8 51 http content 8 1 51 RETROVIROLOGY RESEARCH Open Access The HIV-1 Rev RRE system is required for HIV-1 5 UTR cis elements to augment encapsidation of heterologous RNA into HIV-1 viral particles 1 2 2 1 f 1 Adam S Cockrell Henriette van Praag Nicholas Santistevan Hong Ma 1 and Tal Kafri Abstract Background The process of HIV-1 genomic RNA gRNA encapsidation is governed by a number of viral encoded components most notably the Gag protein and gRNA cis elements in the canonical packaging signal y . Also implicated in encapsidation are cis determinants in the R U5 and PBS primer binding site from the 5 untranslated region UTR . Although conventionally associated with nuclear export of HIV-1 RNA there is a burgeoning role for the Rev RRE in the encapsidation process. Pleiotropic effects exhibited by these cis and trans viral components may confound the ability to examine their independent and combined impact on encapsidation of RNA into HIV-1 viral particles in their innate viral context. We systematically reconstructed the HIV-1 packaging system in the context of a heterologous murine leukemia virus MLV vector RNA to elucidate a mechanism in which the Rev RRE system is central to achieving efficient and specific encapsidation into HIV-1 viral particles. Results We show for the first time that the Rev RRE system can augment RNA encapsidation independent of all cis elements from the 5 UTR R U5 PBS and y . Incorporation of all the 5 UTR cis elements did not enhance RNA encapsidation in the absence of the Rev RRE system. In fact we demonstrate that the Rev RRE system is required for specific and efficient encapsidation commonly associated with the canonical packaging signal. The mechanism of Rev RRE-mediated encapsidation is not a general phenomenon since the combination of the Rev RRE system and 5 UTR cis elements did not enhance encapsidation into MLV-derived viral particles. Lastly we show that heterologous MLV
