Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Polarized expression of the membrane ASP protein derived from HIV-1 antisense transcription in T cells. | RETROVIROLOGY Polarized expression of the membrane ASP protein derived from HIV-1 antisense transcription in T cells Clerc et al. Clerc et al. Retrovirology 2011 8 74 http content 8 1 74 19 September 2011 BioMed Central Clerc et al. Retrovirology 2011 8 74 http content 8 1 74 RESEARCH RETR0VIR0L0GY Open Access Polarized expression of the membrane ASP protein derived from HIV-1 antisense transcription in T cells I I Z I IS-. f I f V 1 2 3t C 1 3 I -s zZ l I im1 2 3 zz I I z rr ZM- i I I - 41 c zk r f I Z I s Z l r z4 5 c I Z D s r Z I 1 2 3 Isabelle Clerc Sylvain Laverdure Cyntnia lorresilla Sébastien Landry Sophie Borel 4 123 123 A I z 123 Amandine Vargas Charlotte Arpin-André Bernard Gay Laurence Briant Antoine Gross Benoit Barbeau4 and Jean-Michel Mesnard1 2 3 Abstract Background Retroviral gene expression generally depends on a full-length transcript that initiates in the 5 LTR which is either left unspliced or alternatively spliced. We and others have demonstrated the existence of antisense transcription initiating in the 3 LTR in human lymphotropic retroviruses including HTLV-1 HTLV-2 and HIV-1. Such transcripts have been postulated to encode antisense proteins important for the establishment of viral infections. The antisense strand of the HIV-1 proviral DNA contains an ORF termed asp coding for a highly hydrophobic protein. However although anti-ASP antibodies have been described to be present in HIV-1-infected patients its in vivo expression requires further support. The objective of this present study was to clearly demonstrate that ASP is effectively expressed in infected T cells and to provide a better characterization of its subcellular localization. Results We first investigated the subcellular localization of ASP by transfecting Jurkat T cells with vectors expressing ASP tagged with the Flag epitope to its N-terminus. Using immunofluorescence microscopy we found that ASP localized to the plasma .
