Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: The early responses of VEGF and its receptors during acute lung injury: implication of VEGF in alveolar epithelial cell survival. | Available online http content 10 5 R130 Research The early responses of VEGF and its receptors during acute lung injury implication of VEGF in alveolar epithelial cell survival Marco Mura Bing Han Cristiano F Andrade Rashmi Seth David Hwang Thomas K Waddell Shaf Keshavjee and Mingyao Liu Thoracic Surgery Research Laboratories Toronto General Research Institute University Health Network Department of Surgery Faculty of Medicine University of Toronto 200 Elizabeth Street Toronto Canada M5G 2C4 Corresponding author Mingyao Liu Received 3 Jun 2006 Revisions requested 21 Jun 2006 Revisions received 17 Jul 2006 Accepted 13 Sep 2006 Published 13 Sep 2006 Critical Care 2006 10 R130 doi cc5042 This article is online at http content 10 5 R130 2006 Mura et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction The function of the vascular endothelial growth factor VEGF system in acute lung injury ALI is controversial. We hypothesized that the role of VEGF in ALI may depend upon the stages of pathogenesis of ALI. Methods To determine the responses of VEGF and its receptors during the early onset of ALI C57BL6 mice were subjected to intestinal ischemia or sham operation for 30 minutes followed by intestinal ischemia-reperfusion IIR for four hours under low tidal volume ventilation with 100 oxygen. The severity of lung injury expression of VEGF and its receptors were assessed. To further determine the role of VEGF and its type I receptor in lung epithelial cell survival human lung epithelial A549 cells were treated with small interference RNA siRNA to selectively silence related genes. Results IIR-induced ALI featured interstitial inflammation .
