Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Homonuclear 1H NMR and circular dichroism study of the HIV-1 Tat Eli variant. | Retrovirology BioMed Central Research Homonuclear 1H NMR and circular dichroism study of the HIV-I Tat Eli variant Jennifer D Watkins1 Grant R Campbell2 Hubert Halimi1 and Erwann P Loret 1 Address 1Unité mixte de recherche Université de la Méditerranée INSERM U911 Faculté de Pharmacie Université de la Méditerranée 27 Boulevard Jean Moulin 13385 Marseille France and 2Department of Pediatrics Division of Infectious Diseases University of California San Diego La Jolla California USA Email Jennifer D Watkins - Grant R Campbell - gcampbel@ Hubert Halimi - Erwann P Loret - Corresponding author Open Access Published 22 September 2008 Received 30 April 2008 Accepted 22 September 2008 Retrovirology 2008 5 83 doi l742-4690-5-83 This article is available from http content 5 1 83 2008 Watkins et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The HIV-1 Tat protein is a promising target to develop AIDS therapies particularly vaccines due to its extracellular role that protects HIV-1-infected cells from the immune system. Tat exists in two different lengths 86 or 87 residues and 99 or 101 residues with the long form being predominant in clinical isolates. We report here a structural study of the 99 residue Tat Eli variant using 2D liquid-state NMR molecular modeling and circular dichroism. Results Tat Eli was obtained from solid-phase peptide synthesis and the purified protein was proven biologically active in a trans-activation assay. Circular dichroism spectra at different temperatures up
