Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Selective translational repression of HIV-1 RNA by Sam68DeltaC occurs by altering PABP1 binding to unspliced viral RNA. | Retrovirology BioMed Central Research Selective translational repression of HIV-1 RNA by Sam68DeltaC occurs by altering PABP1 binding to unspliced viral RNA Kim Marsh1 Vanessa Soros1 2 and Alan Cochrane 1 Address Department of Molecular Genetics University of Toronto Toronto Ontario Canada and 2Sea Lane Biotechnologies 1455 Adams Drive Menlo Park California 94025 USA Email Kim Marsh - Vanessa Soros - vsoros@ Alan Cochrane - Corresponding author Open Access Published 28 October 2008 Received 8 January 2008 Retrovirology 2008 5 97 doi 1742-4690-5-97 Accepted 28 October 2008 This article is available from http content 5 1 97 2008 Marsh et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract HIV-1 structural proteins are translated from incompletely spliced 9 kb and 4 kb mRNAs which are transported to the cytoplasm by Crm1. It has been assumed that once in the cytoplasm translation of incompletely spliced HIV-1 mRNAs occurs in the same manner as host mRNAs. Previous analyses have demonstrated that Sam68 and a mutant thereof Sam68AC have dramatic effects on HIV gene expression strongly enhancing and inhibiting viral structural protein synthesis respectively. While investigating the inhibition of incompletely spliced HIV-1 mRNAs by Sam68AC we determined that the effect was independent of the perinuclear bundling of the viral RNA. Inhibition was dependent upon the nuclear export pathway used as translation of viral RNA exported via the Tap CTE export pathway was not blocked by Sam68AC. We demonstrate that inhibition of HIV expression by Sam68AC is correlated
