Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Lentiviral vector design using alternative RNA export elements. | Retrovirology BioMed Central Research Open Access Lentiviral vector design using alternative RNA export elements Taekeun Oh13 Ali Bajwa14 Guangfu Jia1 and Frank Park 1 2 Address department of Medicine Kidney Disease Center Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee WI USA department of Physiology Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee WI USA department of Internal Medicine Chungbuk National University Hospital Cheongju South Korea and department of Medicine Gene Therapy Program Louisiana State University Health Sciences Center 533 Bolivar St. New Orleans LA USA Email Taekeun Oh - tgohkjs@ Ali Bajwa - abajwa@ Guangfu Jia - jguangfu@ Frank Park - fpark@ Corresponding author fEqual contributors Published 5 June 2007 Received 21 February 2007 _ . Accepted 5 June 2007 Retrovirology 2007 4 38 doi 1742-4690-4-38 This article is available from http content 4 1 38 2007 Oh et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Lentiviral vectors have been designed with complex RNA export sequences in both the integrating and packaging plasmids in order to co-ordinate efficient vector production. Recent studies have attempted to replace the existing complex rev RRE system with a more simplistic RNA export system from simple retroviruses to make these vectors in a rev-independent manner. Results Towards this end lentiviral transfer plasmids were modified with various cis-acting DNA elements that co-ordinate RNA export during viral production to determine their ability to affect the efficiency of vector titer and transduction in different immortalized cell lines in vitro. It was found that .
