Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Characterization of the invariable residue 51 mutations of human immunodeficiency virus type 1 capsid protein on in vitro CA assembly and infectivity. | Retrovirology BioMed Central Research Characterization of the invariable residue 51 mutations of human immunodeficiency virus type I capsid protein on in vitro CA assembly and infectivity Samir Abdurahman11 Masoud Youssefi11 Stefan Hõglund2 and Anders Vahlne 1 Open Access Address Division of Clinical Virology Karolinska Institutet F68 Karolinska University Hospital SE-141 86 Stockholm Sweden and 2Department of Biochemistry Uppsala University Uppsala Sweden Email Samir Abdurahman - Masoud Youssefi - Stefan Hõglund - Anders Vahlne - Corresponding author fEqual contributors Published 28 September 2007 Received 10 August 2007 Accepted 28 September 2007 Retrovirology 2007 4 69 doi l 742-4690-4-69 p p This article is available from http content 4 1 69 2007 Abdurahman et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The mature HIV-1 conical core formation proceeds through highly regulated protease cleavage of the Gag precursor which ultimately leads to substantial rearrangements of the capsid CAp24 molecule involving both inter- and intra-molecular contacts of the CAp24 molecules. In this aspect Asp51 which is located in the N-terminal domain of HIV-1 CAp24 plays an important role by forming a salt-bridge with the free imino terminus Prol following proteolytic cleavage and liberation of the CAp24 protein from the Pr55Gag precursor. Thus previous substitution mutation of Asp51 to alanine D51A has shown to be lethal and that this invariable residue was found essential for tube formation in vitro virus replication and virus capsid formation. Results We extended the above investigation by .
