Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Optimal design and validation of antiviral siRNA for targeting HIV-1. | Retrovirology BioMed Central Open Access Short report Optimal design and validation of antiviral siRNA for targeting HIV-1 Yuki Naito 1 Kyoko Nohtomi2 Toshinari Onogi2 Rie Uenishi2 Kumiko Ui-Tei1 Kaoru Saigo1 and Yutaka Takebe 2 Address Department of Biophysics and Biochemistry Graduate School of Science University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 1130033 Japan and laboratory of Molecular Virology and Epidemiology AIDS Research Center National Institute of Infectious Diseases 1-23-1 Toyama Shinjuku-ku Tokyo 162-8640 Japan Email Yuki Naito - y-naito@ Kyoko Nohtomi - notomi@ Toshinari Onogi - onogit@ Rie Uenishi - uenishir@ Kumiko Ui-Tei - ktei@ Kaoru Saigo - saigo@ Yutaka Takebe - takebe@ Corresponding authors Published 8 November 2007 Received 6 August 2007 Accepted 8 November 2007 Retrovirology 2007 4 80 doi l 742-4690-4-80 This article is available from http content 4 1 80 2007 Naito et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We propose rational designing of antiviral short-interfering RNA siRNA targeting highly divergent HIV-1. In this study conserved regions within HIV-1 genomes were identified through an exhaustive computational analysis and the functionality of siRNAs targeting the highest possible conserved regions was validated. We present several promising antiviral siRNA candidates that effectively inhibited multiple subtypes of HIV-1 by targeting the best conserved regions in pandemic HIV-1 group M strains. Findings RNA interference RNAi is now widely used to knockdown gene expression in a .
