Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity. | Retrovirology BioMed Central Research Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity Niek P van Til Kirstin M Heutinck Roos van der Rijt Coen C Paulusma Michel van Wijland David M Markusic Ronald PJ Oude Elferink and Jurgen Seppen Open Access Address AMC Liver Center Meibergdreef 69 1105 BK. Amsterdam the Netherlands Email Niek P van Til - Kirstin M Heutinck - Roos van der Rijt - Coen C Paulusma - Michel van Wijland - David M Markusic - dmarkusic@ Ronald PJ Oude Elferink - Jurgen Seppen - Corresponding author Published I February 2008 Received 24 October 2007 Accepted 1 February 2008 Retrovirology 2008 5 14 doi 1742-4690-5-14 p This article is available from http content 5 1 14 2008 van Til et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The presence of cholesterol in the Human Immunodeficiency Virus HIV lipid envelop is important for viral function as cholesterol depleted viral particles show reduced infectivity. However it is less well established whether other viral membrane lipids are also important for HIV infection. The ABCB4 protein is a phosphatidyl choline PC floppase that mediates transport of PC from the inner to the outer membrane leaflet. This property enabled us to modulate the lipid composition of HIV vectors and study the effects on membrane composition and infection efficiency. Results Virus generated in the presence of ABCB4 was enriched in PC and cholesterol but contained less sphingomyelin SM . Viral .
