Một nghiên cứu cho thấy rằng Dnmt3a, thông qua miền ATRX tương đồng của nó, trực tiếp tương tác với Suv39h1 (Hình 1; Fuks et al 2003.). Một nghiên cứu khác cho thấy các hoạt động H3-K9 methyltransferase liên quan với Dnmt3b | Establishment and Maintenance of DNA Methylation Patterns in Mammals 193 et al. 2001 Chenet . Such a localization pattern . to be dependent on H3-K9 methylation as Dnmt3b and HP1 fail to concentrate at heterochromatic foci in Suv39hl and Suv39h2 double knockout cells Lehn-ertz et ai. 2003 . Co-IP txperlmentsshow drat Dnmt3a nd Dnmllb bosm complexeewdih HP1 appareoby in aOnveih-inbopendent mnner Fuktei al. 2003 PnhntuPz ton. 2003 . Dnmt3nocdDnmtLbAevhPeobeoo uhown io associatewithH3-Knmethy trrnnfeense ontuvity Fuks eo c. 2OO3 Le-meclz so al. 2003 . kbe siudy uhowslhal. viu ids intetacttwifb Suv39ho iFie-t FuCuotP. . seperote ptudkehph1 that the H3-Kn nei .3b enwiid-type and SentPh double hwoskuut selis arsequLlg oibust suggettiog Dnmt3b formsuneormore histpns-DhA eonlainlng Suv39h-opeeiotpdH3-Ke meWoilrunpSeraseu Lehmetz t p. 20U3i. SUMO-1 OStP OlASti idO PIASpss The smPl ubiquitin-related protein SUMO-1 posttranslationply modifies many proteinswithrolesin UveriA processes including regulation of transcription chromatin structure and DNA repair. SUMO-1 is ligated to lysine residues iosuoslrats proteins pI- i three-step enoymatlcproeossinoo-viwg a heterobinterie ki a liwiimn zy mh LiABl AE-li-nEe hOhiugating enzyme Ubc9 and u nubbor ol ns hunting mn-ames PIAS eroldinSiRng-iih and Pc2 . igm-raot is ubiguilmolio0i SLmoyioiiond es noi promute prolein degradationbut instead mcdulates segeiroi obmrospector0pro-oin3unctihg includinz cobcelldan locPizatiso pro-sio-piotem leíkoDNA interactigh2 uede -hiìliiíiơh ily ll nil 21-U . Using bgaetdilo-hybrid scirehnr wo orouit hnvo -denlldeU setseep colts ponents of th sumsyUatihrmouhinery as D nio-i hkoIl nmIhb-inlu1aeiinp itlo1udoUbo9 OIAS1 antlPỈASxoeThe interactions are further confirmsdgy co-lodPSoalinn 10- I and-ỉgT sell-down expirim-ms. Mutageneo-oanPososmsp tkc .
