Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Immunohistochemical characterization of nodose cough receptor neurons projecting to the trachea of guinea pigs. | Cough BioMed Central Research Immunohistochemical characterization of nodose cough receptor neurons projecting to the trachea of guinea pigs Stuart B Mazzone and Alice E McGovern Open Access Address School of Biomedical Sciences The University of Queensland St Lucia 4072 Australia Email Stuart B Mazzone - Alice E McGovern - Corresponding author Published 19 October 2008 Received 5 September 2008 Cough 2008 4 9 doi l745-9974-4-9 Accepted 19 October 2008 This article is available from http content 4 1 9 2008 Mazzone and McGovern licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Cough in guinea pigs is mediated in part by capsaicin-insensitive low threshold mechanoreceptors cough receptors . Functional studies suggest that cough receptors represent a homogeneous population of nodose ganglia-derived sensory neurons. In the present study we set out to characterize the neurochemical profile of cough receptor neurons in the nodose ganglia. Methods Nodose neurons projecting to the guinea pig trachea were retrogradely labeled with fluorogold and processed immunohistochemically for the expression of a variety of transporters Na K 2C1- co-transporter NKCC1 a1 and a3 Na K ATPase vesicular glutamate transporters vGlut 1 and vGlut2 neurotransmitters substance P calcitonin gene-related peptide CGRP somatostatin neuronal nitric oxide synthase nNOS and cytosolic proteins neurofilament calretinin calbindin parvalbumin . Results Fluorogold labeled 3 per cent of neurons in the nodose ganglia with an average somal perimeter of 137 pm range 90-200 pm . All traced neurons and seemingly all nodose neurons were immunoreactive for NKCC1. Many 90 per cent .
