Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Danaparoid sodium inhibits systemic inflammation and prevents endotoxin-induced acute lung injury in rats. | Available online http content 1 2 2 R43 Research Danaparoid sodium inhibits systemic inflammation and prevents endotoxin-induced acute lung injury in rats Satoshi Hagiwara Hideo Iwasaka Seigo Hidaka Sohei Hishiyama and Takayuki Noguchi Department of Brain and Nerve Science Anesthesiology Oita University Faculty of Medicine Oita Japan Corresponding author Satoshi Hagiwara saku@ Received 27 Nov 2007 Revisions requested 16 Jan 2008 Revisions received 5 Feb 2008 Accepted 2 Apr 2008 Published 2 Apr 2008 Critical Care 2008 12 R43 doi cc6851 This article is online at http content 12 2 R43 2008 Hagiwara et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Systemic inflammatory mediators including high mobility group box 1 HMGB1 play an important role in the development of sepsis. Anticoagulants such as danaparoid sodium DA may be able to inhibit sepsis-induced inflammation but the mechanism of action is not well understood. We hypothesised that DA would act as an inhibitor of systemic inflammation and prevent endotoxin-induced acute lung injury in a rat model. Methods We used male Wistar rats. Animals in the intervention arm received a bolus of 50 U kg of DA or saline injected into the tail vein after lipopolysaccharide LPS administration. We measured cytokine tumour necrosis factor TNF a interleukin IL -6 and IL-10 and HMGB1 levels in serum and lung tissue at regular intervals for 12 h following LPS injection. The mouse macrophage cell line RAW was assessed following stimulation with LPS alone or concurrently with DA with identification of HMGB1 and other cytokines in the supernatant. Results Survival was significantly higher and lung .
