Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: The virion-associated incoming HIV-1 RNA genome is not targeted by RNA interference. | Retrovirology BioMed Central Research Open Access The virion-associated incoming HIV-I RNA genome is not targeted by RNA interference Ellen M Westerhoutt Olivier ter Braket and Ben Berkhout Address Laboratory of Experimental Virology Department of Medical Microbiology Center for Infection and Immunity Amsterdam CINIMA Academic Medical Center University of Amsterdam The Netherlands Email Ellen M Westerhout - Olivier ter Brake - Ben Berkhout - Corresponding author fEqual contributors Published 04 September 2006 Received II July 2006 Accepted 04 September 2006 Retrovirology 2006 3 57 doi 1742-4690-3-57 This article is available from http content 3 1 57 2006 Westerhout et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background RNA interference RNAi has proven to be a powerful tool to suppress gene expression and can be used as a therapeutic strategy against human pathogenic viruses such as human immunodeficiency virus type 1 HIV-1 . Theoretically RNAi-mediated inhibition can occur at two points in the replication cycle upon viral entry before reverse transcription of the RNA genome and on the newly transcribed viral RNA transcripts. There have been conflicting results on whether RNAi can target the RNA genome of infecting HIV-1 particles. We have addressed this issue with HIV-1-based lentiviral vectors. Results We determined the transduction efficiency of a lentiviral vector as measured by GFP expressing cells which reflects the number of successful integration events in a cell line stably expressing shNef. We did not observe a difference in the transduction efficiency comparing lentiviral vectors with or without .
