Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: HIV-2 Protease resistance defined in yeast cells. | Retrovirology BioMed Central Open Access HIV-2 Protease resistance defined in yeast cells Najoua Ben M Barek Gilles Audoly Didier Raoult and Pablo Gluschankof Address Unité des Rickettsies Faculté de Médecine 27 bd Jean Moulin 13385 Marseille cedex 05 Pathologies Transmissibles et Pathologies Infectieuses Tropicales IFR48 France Email Najoua Ben M Barek - Gilles Audoly - Didier Raoult - Pablo Gluschankof - Corresponding author Published 06 September 2006 Received 26 May 2006 Accepted 06 September 2006 Retrovirology 2006 3 58 doi l 742-4690-3-58 This article is available from http content 3 1 58 2006 M Barek et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Inhibitors of the HIV-1 Protease currently used in therapeutic protocols have been found to inhibit although at higher concentrations the HIV-2 encoded enzyme homologue. Similar to observations in HIV-1 infected individuals therapeutic failure has also been observed for some patients infected with HIV-2 as a consequence of the emergence of viral strains resistant to the anti-retroviral molecules. In order to be able to define the specific mutations in the Protease that confer loss of susceptibility to Protease Inhibitors we set up an experimental model system based in the expression of the viral protein in yeast. Results Our results show that the HIV-2 Protease activity kills the yeast cell and this process can be abolished by inhibiting the viral enzyme activity. Since this inhibition is dose dependent IC50 values can be assessed for each anti-retroviral molecule tested. We
