Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Inhibition of PP2A by LIS1 increases HIV-1 gene expression. | Retrovirology BioMed Central Open Access Inhibition of PP2A by LIS1 increases HIV-1 gene expression Nicolas Epie1 2 Tatyana Ammosova1 Willie Turner2 and Sergei Nekhai 1 3 Address 1Center for Sickle Cell Disease Howard University College of Medicine 520 W Street . Washington DC 20059 USA 2Department of Microbiology Howard University College of Medicine 520 W Street . Washington DC 20059 USA and 3Department of Biochemistry and Molecular Biology Howard University College of Medicine 520 W Street . Washington DC 20059 USA Email Nicolas Epie - nepie@ Tatyana Ammosova - tammosova@ Willie Turner - wturner@ Sergei Nekhai - snekhai@ Corresponding author Published 02 October 2006 Received 21 March 2006 Accepted 02 October 2006 Retrovirology 2006 3 65 doi l 742-4690-3-65 This article is available from http content 3 1 65 2006 Epie et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background Lissencephaly is a severe brain malformation in part caused by mutations in the LIS1 gene. LIS1 interacts with microtubule-associated proteins and enhances transport of microtubule fragments. Previously we showed that LIS1 interacts with HIV-1 Tat protein and that this interaction was mediated by WD40 domains of LIS1. In the present study we analyze the effect of LIS1 on Tat-mediated transcription of HIV-1 LTR. Results Tat-mediated HIV-l transcription was upregulated in 293 cells transfected with LISl expression vector. The WD5 but not the N-terminal domain of LIS1 increases Tat-dependent HIV-1 transcription. The effect of LIS1 was similar to the effect of okadaic acid an .
