Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Preferences for the selection of unique tRNA primers revealed from analysis of HIV-1 replication in peripheral blood mononuclear cells. | Retrovirology BioMed Central Research Open Access Preferences for the selection of unique tRNA primers revealed from analysis of HIV-1 replication in peripheral blood mononuclear cells Kenda L Moore-Rigdon1 Barry R Kosloff2 Richard L Kirkman2 and Casey D Morrow 2 Address department of Microbiology University of Alabama at Birmingham Birmingham Alabama 35294 USA and department of Cell Biology University of Alabama at Birmingham Birmingham Alabama 35294 USA Email Kenda L Moore-Rigdon - kendalee@ Barry R Kosloff- retrovir@ Richard L Kirkman - kirkman@ Casey D Morrow - caseym@ Corresponding author Published 24 March 2005 Received 08 February 2005 Accepted 24 March 2005 Retrovirology 2005 2 21 doi 1742-4690-2-21 This article is available from http content 2 1 21 2005 Moore-Rigdon et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background All human immunodeficiency virus HIV-1 uses a host tRNALys 3 as the primer for reverse transcription. The tRNALys 3 is bound to a region on the HIV- 1 genome the primer-binding site PBS that is complementary to the 18 terminal nucleotides of tRNALys 3. How HIV- 1 selects the tRNA from the intracellular milieu is unresolved. Results HIV-1 tRNA primer selection has been investigated using viruses in which the primerbinding site PBS and a sequence within U5 were altered so as to be complementary to tRNAMet tRNAPro or tRNAIle. Analysis of the replication of these viruses in human peripheral blood mononuclear cells PBMC revealed preferences for the selection of certain tRNAs. HIV-1 with the PBS altered to be complementary to tRNAMet with and without the additional mutation in U5 to be complementary to the anticodon of tRNAMet .
