Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: "Quantification of HTLV-I proviral load in experimentally infected rabbits. | Retrovirology BioMed Central Research Open Access Quantification of HTLV-I proviral load in experimentally infected rabbits Tong-Mao Zhao1 Bishop Hague1 David L Caudell2 R Mark Simpson2 and Thomas J Kindt 1 Address 1Molecular and Cellular Immunogenetics Section National Institute of Allergy and Infectious Diseases National Institutes of Health Bldg 50 Room 5515 50 South Drive Bethesda MD 20892 USA and 2Molecular Pathology Unit Center for Cancer Research National Cancer Institute National Institutes of Health Bldg 37 Room 2002 37 Convent Drive Bethesda MD 20892 USA Email Tong-Mao Zhao - tzhao@ Bishop Hague - bhague@ David L Caudell - caudelld@ R Mark Simpson - simpsoma@ Thomas J Kindt - tkindt@ Corresponding author Published 23 May 2005 Received 12 April 2005 Retrovirology 2005 2 34 doi 1742-4690-2-34 Accepted 23 May 2005 This article is available from http content 2 1 34 2005 Zhao et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Levels of proviral load in HTLV-1 infected patients correlate with clinical outcome and are reasonably prognostic. Adaptation of proviral load measurement techniques is examined here for use in an experimental rabbit model of HTLV-1 infection. Initial efforts sought to correlate proviral load with route and dose of inoculation and with clinical outcome in this model. These methods contribute to our continuing goal of using the model to test treatments that alleviate virus infection. Results A real-time PCR assay was used to measure proviral load in blood and tissue samples from a series of rabbits infected using HTLV-1 inocula prepared as either cell-free virus particles infected .
