Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài:TRIM5α selectively binds a restriction-sensitive retroviral capsid | Retrovirology BioMed Central Short report Open Access TRIM5a selectively binds a restriction-sensitive retroviral capsid Sarah Sebastian and Jeremy Luban Address Departments of Microbiology and Medicine Columbia University College of Physicians and Surgeons 701 West 168th Street HHSC 1502 New York New York 10032 uSa Email Sarah Sebastian - ss2265@ Jeremy Luban - jl45@ Corresponding author Published 20 June 2005 Received 06 June 2005 Retrovirology 2005 2 40 doi 1742-4690-2-40 Accepted 20 June 2005 This article is available from http content 2 1 40 2005 Sebastian and Luban licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract TRIM5 is a potent retrovirus inhibitor that targets viruses bearing particular capsid CA residues. In most primate species retroviral restriction requires the C-terminal SPRY domain unique to the a-isoform of TRIM5 but the mechanism by which susceptible viruses are recognized and targeted for restriction is unknown. Here we show that TRIM5a binds retroviral CA from detergentstripped virions in a SPRY-dependent manner with sufficient discrimination to account for the exquisite specificity of restriction. Findings Two independent screens identified TRIM5 as a potent retrovirus restriction element that targets select viruses after entry into primate cells 1 2 . The biochemical basis for specificity of restriction is only evident in cells of the owl monkey where HIV-1 CA is recognized by the C-terminal cyclophilin domain that is unique to the TRIM5 orthologue found in this genus 2-4 . In all other primates including humans and macaques potent CA-specific restriction is conferred by the TRIM5a isoform 1 5-9 which possesses a C-terminal SPRY domain 10 .
