Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài:" The pp24 phosphoprotein of Mason-Pfizer monkey virus contributes to viral genome packaging | Retrovirology BioMed Central Research The pp24 phosphoprotein of Mason-Pfizer monkey virus contributes to viral genome packaging Christopher R Bohl Shanna M Brown and Robert A Weldon Jr Open Access Address School of Biological Sciences and the Nebraska Center for Virology University of Nebraska Lincoln 68588 USA Email Christopher R Bohl - cbohl1@ Shanna M Brown - shanghai001@ Robert A Weldon - rweldon2@ Corresponding author Published 07 November 2005 Received 12 April 2005 Accepted 07 November 2005 Retrovirology 2005 2 68 doi 1742-4690-2-68 H This article is available from http content 2 1 68 2005 Bohl et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The Gag protein of Mason-Pfizer monkey virus a betaretrovirus contains a phosphoprotein that is cleaved into the Np24 protein and the phosphoprotein pp16 18 during virus maturation. Previous studies by Yasuda and Hunter J. Virology. 1998. 72 4095-4103 have demonstrated that pp16 18 contains a viral late domain required for budding and that the Np24 protein plays a role during the virus life cycle since deletion of this N-terminal domain blocked virus replication. The function of the Np24 domain however is not known. Results Here we identify a region of basic residues KKPKR within the Np24 domain that is highly conserved among the phosphoproteins of various betaretroviruses. We show that this KKPKR motif is required for virus replication yet dispensable for procapsid assembly membrane targeting budding and release particle maturation or viral glycoprotein packaging. Additional experiments indicated that deletion of this motif reduced viral RNA packaging 6-8 fold and affected the transient .
