Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells | Retrovirology BioMed Central Research Open Access Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells Xiaochuan Chen Jin He and Lung-Ji Chang Address Department of Molecular Genetics and Microbiology Powell Gene Therapy Center McKnight Brain Institute University of Florida College of Medicine Gainesville FL 32610-0266 USA Email Xiaochuan Chen - xchen@ Jin He - jinhe@ Lung-Ji Chang - lchang@ Corresponding author Published 01 November 2004 Received 28 June 2004 Accepted 01 November 2004 Retrovirology 2004 1 37 doi l742-4690-l-37 This article is available from http content l l 37 2004 Chen et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Dendritic cells DCs are professional antigen-presenting cells that play important roles during human immunodeficiency virus type l HIV-l infection. HIV-l derived lentiviral vectors LVs transduce DCs at high efficiency but their effects on DC functions have not been carefully studied. Modification of DCs using LVs may lead to important applications in transplantation treatment of cancer autoimmune and infectious diseases. Results Using DCs prepared from multiple blood donors we report that LV transduction of DCs resulted in altered DC phenotypes and functions. Lentiviral transduction of DCs resulted in downregulation of cell surface molecules including CDla co-stimulatory molecules CD80 CD86 ICAM-l and DC-SIGN. DCs transduced with LVs displayed a diminished capacity to polarize naive T cells to differentiate into Thl effectors. This impaired Thl response could be fully corrected by cotransduction of DCs with LVs encoding interleukin-l2 IL-12 interferon-gamma IFN-y or .
