Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: MHC class I expression protects rat colon carcinoma cells from hepatic natural killer cell-mediated apoptosis and cytolysis, by blocking the perforin/granzyme pathway. | Comparative Hepatology BioMed Central Research Open Access MHC class I expression protects rat colon carcinoma cells from hepatic natural killer cell-mediated apoptosis and cytolysis by blocking the perforin granzyme pathway Dianzhong Luot1 3 David Vermijlent 1 Peter JK Kuppen2 and Eddie Wisse1 Address Laboratory for Cell Biology and Histology Vrije Universiteit Brussel VUB Laarbeeklaan 103 1090 Brussels Belgium 2Department of Surgery Leiden University Medical Center 2300 RC Leiden The Netherlands and 3Department of Pathology Guangxi Medical University Nanning China E-mail Dianzhong Luo - dianzhongluo@ David Vermijlen - dvermijl@ Peter JK Kuppen - Kuppen@ Eddie Wisse - wisse@ Corresponding author tEqual contributors Published 20 November 2002 Received 28 August 2002 Accepted 20 NOvember 2002 Comparative Hepatology 2002 1 2 This article is available from http content 1 1 2 2002 Luo et al licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Background Hepatic natural killer NK cells the most cytotoxic cells of the natural occurring NK cells are located in the liver sinusoids and are thus in a strategic position to kill arriving metastasising tumour cells like colon carcinoma cells. It is known that major histocompatibility complex MHC class I on tumour cells negatively regulates NK cell-mediated cytolysis but this is found using blood- or spleen-derived NK cells. Therefore using isolated rat hepatic NK cells and the syngeneic colon carcinoma cell line CC53ls we investigated whether this protective role of MHC class I is also operative in hepatic NK cells and addressed the mechanism of MHC class I protection. Results When MHC class I on CC531s cells was masked by preincubation with monoclonal antibody OX18 hepatic
