Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Response of sinusoidal mouse liver cells to choline-deficient ethionine-supplemented diet. | Ueberham et al. Comparative Hepatology 2010 9 8 http content 9 1 8 COMPARATIVE HEPATOLOGY RESEARCH Open Access Response of sinusoidal mouse liver cells to choline-deficient ethionine-supplemented diet Elke Ueberham1 Jan Bottger1 Uwe Ueberham2 4 Jens Grosche3 4 Rolf Gebhardt1 Abstract Background Proliferation of oval cells the bipotent precursor cells of the liver requires impeded proliferation and loss of hepatocytes as well as a specific micro-environment provided by adjacent sinusoidal cells of liver. Despite their immense importance for triggering the oval cell response cells of hepatic sinusoids are rarely investigated. To elucidate the response of sinusoidal liver cells we have employed a choline-deficient ethionine-supplemented CDE diet a common method for inducing an oval cell response in rodent liver. We have utilised selected expression markers commonly used in the past for phenotypic discrimination of oval cells and sinusoidal cells cytokeratin E-cadherin and M2-pyruvate kinase for oval cells and glial fibrillary acidic protein GFAP was used for hepatic stellate cells HSCs . Results CDE diet leads to an activation of all cells of the hepatic sinusoid in the mouse liver. Beside oval cells also HSCs and Kupffer cells proliferate. The entire fraction of proliferating cells in mouse liver as well as endothelial cells and cholangiocytes express M2-pyruvate kinase. Concomitantly GFAP long considered a unique marker of quiescent HSCs was upregulated in activated HSCs and expressed also in cholangiocytes and oval cells. Conclusions Our results point to an important role of all types of sinusoidal cells in regeneration from CDE induced liver damage and call for utmost caution in using traditional marker for identifying specific cell types. Thus M2-pyruvate kinase should no longer be used for estimating the oval cell response in mouse liver. CDE diet leads to activation of GFAP positive HSCs in the pericentral zone of liver lobulus. .
