Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Two-dimensional difference gel electrophoresis (DIGE) analysis of sera from visceral leishmaniasis patients. | CLINICAL PROTEOMICS Two-dimensional difference gel electrophoresis DIGE analysis of sera from visceral leishmaniasis patients Rukmangadachar et al. Rukmangadachar et al. Clinical Proteomics 2011 8 4 http content 8 1 4 31 May 2011 BioMed Central Rukmangadachar et al. Clinical Proteomics 2011 8 4 http content 8 1 4 CLINICAL PROTEOMICS RESEARCH Open Access Two-dimensional difference gel electrophoresis DIGE analysis of sera from visceral leishmaniasis patients Lokesh A Rukmangadachar1 2 Jitender Kataria1 Gururao Hariprasad1 Jyotish C Samantaray3 and Alagiri Srinivasan1 Correspondence srini@ department of Biophysics All India Institute of Medical Sciences New Delhi 110029 India Full list of author information is available at the end of the article Abstract Introduction Visceral leishmaniasis is a parasitic infection caused by Lesihmania donovani complex and transmitted by the bite of the phlebotomine sand fly. It is an endemic disease in many developing countries with more than 90 of the cases occurring in Bangladesh India Nepal Sudan Ethiopia and Brazil. The disease is fatal if untreated. The disease is conventionally diagnosed by demonstrating the intracellular parasite in bone marrow or splenic aspirates. This study was carried out to discover differentially expressed proteins which could be potential biomarkers. Methods Sera from six visceral leishmaniasis patients and six healthy controls were depleted of high abundant proteins by immunodepletion. The depleted sera were compared by 2-D Difference in gel electrophoresis DIGE . Differentially expressed proteins were identified the by tandem mass spectrometry. Three of the identified proteins were further validated by western blotting. Results This is the first report of serum proteomics study using quantitative Difference in gel electrophoresis DIGE in visceral leishmaniasis. We identified alpha-1-acidglycoprotein and C1 inhibitor as up .
