Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: The basophil activation test by flow cytometry: recent developments in clinical studies, standardization and emerging perspectives. | Clinical and Molecular Allergy BioMed Central Review Open Access The basophil activation test by flow cytometry recent developments in clinical studies standardization and emerging perspectives Radhia Boumiza1 Anne-Lise Debard1 and Guillaume Monneret 1 2 Address 1Immunology Laboratory Lyon-Sud University Hospital Lyon France and 2Immunology Laboratory Hôpital Neurologique Lyon France Email Radhia Boumiza- radhiaboumiza@ Anne-Lise Debard - Guillaume Monneret - Corresponding author Published 30 June 2005 Received 04 May 2005 Clinical and Molecular Allergy 2005 3 9 doi 86 1476-7961-3-9 Accepted 30 June 2005 This article is available from http content 3 1 9 2005 Boumiza et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The diagnosis of immediate allergy is mainly based upon an evocative clinical history positive skin tests gold standard and if available detection of specific IgE. In some complicated cases functional in vitro tests are necessary. The general concept of those tests is to mimic in vitro the contact between allergens and circulating basophils. The first approach to basophil functional responses was the histamine release test but this has remained controversial due to insufficient sensitivity and specificity. During recent years an increasing number of studies have demonstrated that flow cytometry is a reliable tool for monitoring basophil activation upon allergen challenge by detecting surface expression of degranulation activation markers CD63 or CD203c . This article reviews the recent improvements to the basophil activation test made possible by flow cytometry focusing on the use of anti-CRTH2
