Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Sequence homology: A poor predictive value for profilins cross-reactivity. | Clinical and Molecular Allergy BioMed Central Research Sequence homology A poor predictive value for profilins cross-reactivity Mojtaba Sankian1 Abdolreza Varasteh 1 Nazanin Pazouki1 and Mahmoud Mahmoudi2 Open Access Address 1Immunobiochemistry Lab Immunology Research Center Bu-Ali Research Institute Mashhad Iran and 2Molecular biology Lab Immunology Research Center Bu-Ali Research Institute Mashhad Iran Email Mojtaba Sankian - m_sankian@ Abdolreza Varasteh - a-varasteh@ Nazanin Pazouki - npazouki@ Mahmoud Mahmoudi - Mahmoudi@ Corresponding author Published 10 September 2005 Received 28 June 2005 I IA ÍI .7 7A I IAccepted 10 September 2005 Clinical and Molecular Allergy 2005 3 13 doi 1476-7961-3-13 This article is available from http 1476-7961 3 1 3 2005 Sankian et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Summary Background Profilins are highly cross-reactive allergens which bind IgE antibodies of almost 20 of plant-allergic patients. This study is aimed at investigating cross-reactivity of melon profilin with other plant profilins and the role of the linear and conformational epitopes in human IgE cross-reactivity. Methods Seventeen patients with melon allergy were selected based on clinical history and a positive skin prick test to melon extract. Melon profilin has been cloned and expressed in E. coli. The IgE binding and cross-reactivity of the recombinant profilin were measured by ELISA and inhibition ELISA. The amino acid sequence of melon profilin was compared with other profilin sequences. A combination of chemical cleavage and immunoblotting techniques were used to define the role of conformational and linear epitopes in IgE .
