Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: House dust mite major allergens Der p 1 and Der p 5 activate human airway-derived epithelial cells by protease-dependent and protease-independent mechanisms. | Clinical and Molecular Allergy BioMed Central Research Open Access House dust mite major allergens Der p 1 and Der p 5 activate human airway-derived epithelial cells by protease-dependent and protease-independent mechanisms Henk F Kauffman1 Michael Tamm2 J André B Timmerman1 and Peter Borger 2 Address department of Allergology University Medical Centre Groningen Hanzeplein 1 Groningen The Netherlands and 2Pulmonary Cell Research University Hospital Basel Hebelstrasse 20 Basel Switzerland Email Henk F Kauffman - Michael Tamm - mtamm@ J André B Timmerman - Peter Borger - Corresponding author Published 28 March 2006 Received 04 October 2005 Accepted 28 March 2006 Clinical and Molecular Allergy 2006 4 5 doi 1476-7961-4-5 r This article is available from http content 4 1 5 2006 Kauffman et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract House dust mite allergens HDM cause bronchoconstriction in asthma patients and induce an inflammatory response in the lungs due to the release of cytokines chemokines and additional mediators. The mechanism how HDM components achieve this is largely unknown. The objective of this study was to assess whether HDM components of Dermatophagoides pteronissinus with protease activity Der p 1 and unknown enzymatic activity Der p 2 Der p 5 induce biological responses in a human airway-derived epithelial cell line A549 and if so to elucidate the underlying mechanism s of action. A549 cells were incubated with HDM extract Der p I recombinant Der p 2 and recombinant Der p 5. Cell desquamation was assessed by microscopy. The proinflammatory cytokines IL-6 and IL-8 were measured .
