Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: The identification of allergen proteins in sugar beet (Beta vulgaris) pollen causing occupational allergy in greenhouses. | Clinical and Molecular Allergy BioMed Central Research The identification of allergen proteins in sugar beet Beta vulgaris pollen causing occupational allergy in greenhouses Susanne Luoto1 Wietske Lambert2 Anna Blomqvist1 and Cecilia Emanuelsson 2 Open Access Address Occupational and Environmental medicine County Hospital Halmstad Sweden and 2Department of Biochemistry Lund University Lund Sweden Email Susanne Luoto - Wietske Lambert - Anna Blomqvist - Cecilia Emanuelsson - Corresponding author Published II August 2008 Received 18 January 2008 Accepted 1 1 August 2008 Clinical and Molecular Allergy 2008 6 7 doi 86 1476-796 1-6-7 This article is available from http content 6 1 7 2008 Luoto et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background During production of sugar beet Beta vulgaris seeds in greenhouses workers frequently develop allergic symptoms. The aim of this study was to identify and characterize possible allergens in sugar beet pollen. Methods Sera from individuals at a local sugar beet seed producing company having positive SPT and specific IgE to sugar beet pollen extract were used for immunoblotting. Proteins in sugar beet pollen extracts were separated by 1- and 2-dimensional electrophoresis and IgE-reactive proteins analyzed by liquid chromatography tandem mass spectrometry. Results A 14 kDa protein was identified as an allergen since IgE-binding was inhibited by the well-characterized allergen Che a 2 profilin from the related species Chenopodium album. The presence of 17 kDa and 14 kDa protein homologues to both the .
