Clinical and Molecular Allergy Research Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Mapping of IgE-binding regions on recombinant Cyn d 1, a major allergen from Bermuda Grass Pollen (BGP). | Clinical and Molecular Allergy BioMed Central Research Mapping of IgE-binding regions on recombinant Cyn d 1 a major allergen from Bermuda Grass Pollen BGP Ruby Tiwari1 2 Prem L Bhalla1 and Mohan B Singh 1 Open Access Address 1ARC Centre of Excellence for Integrative Legume Research Faculty of Land and Food Resources University of Melbourne Parkville Australia and institute of Food Nutrition and Human Health Massey University Palmerston North New Zealand Email Ruby Tiwari - rtiwari14@ Prem L Bhalla - premlb@ Mohan B Singh - mohan@ Corresponding author Published 2 February 2009 Received 22 September 2008 . . .__. n Accepted 2 February 2009 Clinical and Molecular Allergy 2009 7 3 doi 1476-7961-7-3 This article is available from http content 7 1 3 2009 Tiwari et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background Bermuda grass Cynodon dactylon subfamily Chloridoideae is an important source of seasonal aeroallergens in warm tropical and sub-tropical areas worldwide. Improved approaches to diagnosis and therapy of allergic diseases require a thorough understanding of the structure and epitopes on the allergen molecule that are crucial for the antigen-antibody interaction. This study describes the localization of the human IgE-binding regions of the major group I pollen allergen Cyn d 1 from Bermuda grass. Methods A cDNA library was constructed from Bermuda grass pollen BGP using a Lambda gt11 expression vector. The gene encoding the Cyn d 1 allergen was isolated by screening the library with a mouse monoclonal antibody raised against grass group 1 .
