Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Interleukin 2-regulated in vitro antibody production following a single spinal manipulative treatment in normal subjects. | Teodorczyk-Injeyan et al. Chiropractic Osteopathy 2010 18 26 http content 18 1 26 CHIROPRACTIC OSTEOPATHY RESEARCH Open Access Interleukin 2-regulated in vitro antibody production following a single spinal manipulative treatment in normal subjects Julita A Teodorczyk-Injeyan1 Marion McGregor2 Richard Ruegg3 H Stephen Injeyan4 Abstract Background Our recent investigations have demonstrated that cell cultures from subjects who received a single spinal manipulative treatment in the upper thoracic spine show increased capacity for the production of the key immunoregulatory cytokine interleukin-2. However it has not been determined if such changes influence the response of the immune effector cells. Thus the purpose of the present study was to determine whether in the same subjects spinal manipulation-related augmentation of the in vitro interleukin-2 synthesis is associated with the modulation of interleukin 2-dependent and or interleukin-2-induced humoral immune response antibody synthesis . Methods A total of seventy-four age and sex-matched healthy asymptomatic subjects were studied. The subjects were assigned randomly to venipuncture control n 22 spinal manipulative treatment without cavitation n 25 or spinal manipulative treatment associated with cavitation n 27 groups. Heparinized blood samples were obtained from the subjects before baseline and then at 20 minutes and 2 hours post-treatment. Immunoglobulin antibody synthesis was induced in cultures of peripheral blood mononuclear cells by stimulation with conventional pokeweed mitogen or by application of human recombinant interleukin-2. Determinations of the levels of immunoglobulin G and immunoglobulin M production in culture supernatants were performed by specific immunoassays. Results The baseline levels of immunoglobulin synthesis induced by pokeweed mitogen or human recombinant interleukin-2 stimulation were comparable in all groups. No significant changes in the production of pokeweed
