Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Osteoprotective effects of Fructus Ligustri Lucidi aqueous extract in aged ovariectomized rats. | Ko et al. Chinese Medicine 2010 5 39 http content 5 1 39 CHINESE MEDICINE RESEARCH Open Access Osteoprotective effects of Fructus Ligustri Lucidi aqueous extract in aged ovariectomized rats Chun Hay Ko1 Wing Sum Siu1 Ching Po Lau1 Clara Bik San Lau1 Kwok Pui Fung1 2 Ping Chung Leung1 Abstract Background Fructus Ligustri Lucidi FLL is a commonly used herb for treating bone disorders in Chinese medicine. The present study investigates the anti-osteoporotic activity of FLL aqueous extract in the model of postmenopausal bone loss in aged ovariectomized OVX female rats. Methods After eight weeks of treatment of FLL or water the lumbar spine was scanned by peripheral quantitative computed tomography pQCT . Effects of FLL water extract on osteogenic and adipogenic differentiations in rat mesenchymal stem cells MSCs were assessed by biochemical methods and staining. Results FLL aqueous extract significantly inhibited bone mineral density BMD loss in total trabecular and cortical bones without affecting body weight and uterus wet weight. FLL extract significantly promoted osteogenesis and suppressed adipogenesis in MSCs as indicated by the elevated alkaline phosphatase activity calcium deposition levels and decreased adipocyte number in a dose-dependent manner without cytotoxic effects. Real-time PCR analysis revealed significant increase of osteoprotegerin OPG -to-receptor activator for nuclear factor-KB ligand RANKL mRNA indicating a decrease in osteoclastogenesis. Conclusion The present study demonstrates the osteoprotective effects of FLL aqueous extract on aged OVX rats stimulation of osteogenesis inhibition of adipogenesis and osteoclastogenesis in MSCs. Background Fructus Ligustri Lucidi Nuzhenzi FLL the fruit of Ligustrum lucidum is a major herb for treating aged-related diseases 1 . Our previous study demonstrated that an anti-osteoporosis herbal formula containing Herba Epimedii Fructus Ligustri Lucidi and Fructus Psoraleae at a ratio of 5 4 1 .
