Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Anti-proliferative effects of raw and steamed extracts of Panax notoginseng and its ginsenoside constituents on human liver cancer cells. | Toh et al. Chinese Medicine 2011 6 4 http content 6 1 4 CHINESE MEDICINE RESEARCH Open Access Anti-proliferative effects of raw and steamed extracts of Panax notoginseng and its ginsenoside constituents on human liver cancer cells 1 1 1 2 3 1 Ding-Fung Toh Dhavalkumar Narendrabhai Patel Eric Chun-Yong Chan Alvin Teo Soek-Ying Neo Hwee-Ling Koh Abstract Background Panax notoginseng is a potential source of anticancer compounds. This study aims to investigate the effects of steaming on the chemical profile of P. notoginseng and the anti-proliferative effects of P. notoginseng on liver cancer cells. Methods Samples of powdered raw P. notoginseng roots were steamed for various durations. Extracts of the raw and steamed samples were subjected to ultra-high pressure liquid chromatography mass spectrometry UHPLC-MS analysis for chemical profiling. The anti-proliferative effects on three human liver cancer cells namely SNU449 SNU182 and HepG2 were evaluated using colorimetric WST-1 assay. Results Steaming changed chromatographic and pharmacological profiles of P. notoginseng causing differences in activities such as inhibition of cancer growth. Steamed P. notoginseng exhibited greater anti-proliferative effects against liver cancer cells SNU449 SNU182 and HepG2 than its raw form steaming up to 24 hours increased bioactivities. Steaming increased the concentrations of ginsenoside Rh2 Rk1 Rk3 and 20S-Rg3 and enhanced growth inhibition of liver cancer cells. Conclusion Steaming changes the chemical profile as well as anti-cancer biological activities of P. notoginseng. Steamed P. notoginseng contains potential compounds for the treatment of liver cancer. Background Some Chinese medicinal herbs exhibit anti-tumour activities 1 2 . The raw form of Panax notoginseng Burk. . Chen Sanqi is used in Chinese medicine to arrest internal and external haemorrhages eliminate blood stasis improve blood circulation disperse bruises reduce swelling and pain 3 . The .
