Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Ginkgo biloba extract (EGb761) inhibits mitochondria-dependent caspase pathway and prevents apoptosis in hypoxia-reoxygenated cardiomyocytes. | Shen et al. Chinese Medicine 2011 6 8 http content 6 1 8 CHINESE MEDICINE RESEARCH Open Access Ginkgo biloba extract EGb761 inhibits mitochondria-dependent caspase pathway and prevents apoptosis in hypoxia-reoxygenated cardiomyocytes Jiangang Shen 1 Waisin Lee1 Yong Gu1 Yao Tong1 Peter CW Fung2 Li Tong3 Abstract Background EGb761 is a standard extract from the leaves of Ginkgo biloba Yinxing containing ginkgo-flavone glycosides and terpenoid. The flavonoid components of EGb761 scavenge free radicals and protect myocardia from ischemia-reperfusion injury. The present study aims to determine the effects of the active compounds of EGb761 on mitochondria-dependent caspase pathway. Methods Cardiomyocytes were exposed to 24 hours of hypoxia and four hours of reoxygenation and pretreated with EGb761 bilobalide and quertcetin. By using immunoblot immunofluorescent biochemical and flow cytometry techniques we compared the effects of EGb761 and its representative constituents including quercetin and bilobalides on regulating mitochondria-dependent caspases signal pathway and apoptotic cell death in the hypoxia-reoxygenated cardiomyocytes. Results Pretreatment with EGb761 significantly inhibited the release of cytochrome c from mitochondria the expression of caspase-3 cleavage activities of caspases and attenuated apoptotic cell death. The effects of quercetin on the release of cytochrome c the cleavage activities of caspases and cell death were similar to those of EGb761 but better than those of bilobalide. Conclusion The antioxidant constituents of EGb761 such as quercetin contribute to the cardioprotective effects of EGb761 and inhibit the mitochondria-dependent caspase pathway. It is possible that the mitochondria-dependent caspase pathway may be one of the molecular targets of EGb761 against myocardial ischemia-reperfusion injury. Background EGb761 is a standard extract from the leaves of Ginkgo biloba Yinxing containing 24 ginkgo-flavone glycosides eg
