Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS. | Yao et al. Chinese Medicine 2011 6 9 http content 6 1 9 CHINESE MEDICINE RESEARCH Open Access Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS Hong Yao Peiying Shi Qing Shao Xiaohui Fan Abstract Background Xuesaitong XST injection consisting of total saponins from Panax notoginseng was widely used for the treatment of cardio- and cerebro-vascular diseases in China. This study develops a simple and global quality evaluation method for the quality control of XST. Methods High performance liquid chromatography-ultraviolet detection HPLC-UV was used to identify and quantify the chromatographic fingerprints of the XST injection. Characteristic common peaks were identified using HPLC with photo diode array detection electrospray ionization tandem mass spectrometry HPLC-PDA ESI-MSn . Results Representative fingerprints from ten batches of samples showed 27 common saponins all of which were identified and quantified using ten reference saponins. Conclusion Chemical fingerprinting and quantitative analysis identified most of the common saponins for the quality control of P. notoginseng products such as the XST injection. Background Xuesaitong XST injection consisting of total saponins from Panax notoginseng Sanqi was widely used for the treatment of cardiovascular and cerebrovascular diseases in China. As total saponins including ginsenosides and notoginsenosides in the XST inj ection are its active ingredients quality control of total saponins in the XST injection is critical for its safety efficacy and stability. Single or simultaneous determination of main components of the total saponin extracts from P. notoginseng using high performance liquid chromatography-ultraviolet detection HPLC-UV 1-5 high performance liquid chromatography-evaporative light scattering detection HPLC-ELSD 6 high performance liquid chromatography-mass spectroscopy HPLC-MS 7-13 have been reported but over half of the .
