Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Array-based techniques for fingerprinting medicinal herbs. | Niu et al. Chinese Medicine 2011 6 18 http content 6 1 18 CHINESE MEDICINE REVIEW Open Access Array-based techniques for fingerprinting medicinal herbs Linhai Niu 1 Nitin Mantri1 Chun Guang Li 2 Charlie Xue 2 and Edwin Pang 1 Abstract Poor quality control of medicinal herbs has led to instances of toxicity poisoning and even deaths. The fundamental step in quality control of herbal medicine is accurate identification of herbs. Array-based techniques have recently been adapted to authenticate or identify herbal plants. This article reviews the current array-based techniques eg oligonucleotides microarrays gene-based probe microarrays Suppression Subtractive Hybridization SSH -based arrays Diversity Array Technology DArT and Subtracted Diversity Array SDA . We further compare these techniques according to important parameters such as markers polymorphism rates restriction enzymes and sample type. The applicability of the array-based methods for fingerprinting depends on the availability of genomics and genetics of the species to be fingerprinted. For the species with few genome sequence information but high polymorphism rates SDA techniques are particularly recommended because they require less labour and lower material cost. Background Bioactive compounds in certain medicinal herbs affect cell communication and signalling 1 induce inflammatory responses 2 and help prevent diseases 3 . Chinese medicinal herbs such as ginseng Panax ginseng Dan-shen Salvia miltiorrhiza Korean Mint Agastache rugosa Chinese motherwort Leonurus japonicus are globally recognized for treating human disorders. Currently the global market for medicinal herbs currently is valued over 60 billion a year and growing at an annual rate of 4 . Development and acceptance of herbal medicine are hindered by misidentification and adulteration of medicinal herbs which may lead to loss of therapeutic potency and potential intoxication 5 . Authentication of medicinal herbs ensures .
