Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Antiviral biflavonoids from Radix Wikstroemiae (Liaogewanggen). | Huang et al. Chinese Medicine 2010 5 23 http content 5 1 23 CHINESE MEDICINE RESEARCH Open Access Antiviral biflavonoids from Radix Wikstroemiae Liaogewanggen Weihuan Huang1 Xiaoli Zhang2 3 Yifei Wang4 Wencai Ye2 3 Vincent EC Ooi1 Hau Yin Chung1 and Yaolan Li 2 3 Abstract Background Radix Wikstroemiae is a common Chinese herbal medicine. The ethyl acetate fraction of the ethanolic extract of W indica possesses potent in vitro antiviral activity against respiratory syncytial virus RSV . This study aims to identify the antiviral components of the active fraction. Methods The active fraction of the Radix Wikstroemiae extract was isolated with chromatographic methods such as silica gel Sephadex LH-20 and semi-preparative high performance liquid chromatography HPLC columns. The structures of the isolated compounds were determined based on spectroscopic analyses. The in vitro antiviral activity of the compounds against RSV was tested with the cytopathic effect CPE reduction assay and the 3- 4 5-dimethylthiazol-2-yl -2 5-diphenyltetrazolium bromide MTT method. Results Four biflavonoids namely neochamaejasmin B genkwanol B genkwanol C and stelleranol were isolated and characterized. Genkwanol B genkwanol C and stelleranol which are stereo isomers of spirobiflavonoids showed potent anti-RSV activity whereas neochamaejasmin B did not. Conclusion Neochamaejasmin B genkwanol B genkwanol C and stelleranol were isolated from Radix Wikstroemiae and the complete absolute configurations of five chiral carbons in stelleranol were substantiated for the first time. Furthermore the anti-RSV activity of genkwanol B genkwanol C and stelleranol was reported for the first time. Background The root of Wikstroemia indica Linn. C. A. Mey. Thymelaeaceae Radix Wikstroemiae Liaogewanggen is a Chinese herbal medicine used to treat various inflammatory conditions 1 . W indica tablet an over-the-counter product made from the aqueous extract of Radix Wikstroemiae is commercially .
