Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Liposarcoma cells with aldefluor and CD133 activity have a cancer stem cell potential. | Stratford et al. Clinical Sarcoma Research 2011 1 8 http content 1 1 8 CLINICAL SARCOMA RESEARCH RESEARCH Open Access Liposarcoma cells with aldefluor and CD133 activity have a cancer stem cell potential 1 1 12 1 1 Eva W Stratford Russell Castro Anna Wennerstrom Ruth Holm Else Munthe Silje Lauvrak Bodil Bjerkehagen2 and Ola Myklebost1 3 Abstract Aldehyde dehydrogenase ALDH has recently been shown to be a marker of cancer stem-like cells CSCs across tumour types. The primary goals of this study were to investigate whether ALDH is expressed in liposarcomas and whether CSCs can be identified in the ALDHhigh subpopulation. We have demonstrated that ALDH is indeed expressed in 10 out of 10 liposarcoma patient samples. Using a liposarcoma xenograft model we have identified a small population of cells with an inducible stem cell potential expressing both ALDH and CD133 following culturing in stem cell medium. This potential CSC population which makes up for 0 1-1 7 of the cells displayed increased selfrenewing abilities and increased tumourigenicity giving tumours in vivo from as few as 100 injected cells. Introduction CSCs are described as a small population of tumour cells possessing stem-like properties such as the ability to self-renew as well as to differentiate into more mature cells that make up the bulk of the tumour which usually to some extent resembles normal tissue. These cells are also referred to as tumour initiating 1 . The CSCs are in many aspects similar to normal stem cells and are thought to arise either when normal stem cells gain oncogenic mutations which confer enhanced proliferation and lack of homeostatic control mechanisms or alternatively when a progenitor or differentiated cell acquires mutations conferring de-differentiation to a malignant stem-like cell 2 . Since the integrity of stem cells is of critical importance for the organism several mechanisms that ensure the survival of stem cells have evolved. These .
