Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Propofol: neuroprotection in an in vitro model of traumatic brain injury. | Available online http content 13 2 R61 Research Propofol neuroprotection in an in vitro model of traumatic brain injury Jan Rossaint1 Rolf Rossaint1 Joachim Weis2 Michael Fries1 Steffen Rex3 and Mark Coburn1 Open Access Department of Anesthesiology RWTH Aachen University Hospital PauwelsstraBe 30 52074 Aachen Germany institute of Neuropathology University Hospital of the RWTH Aachen PauwelsstraBe 30 52074 Aachen Germany department of Surgical Intensive Care University Hospital of the RWTH Aachen PauwelsstraBe 30 52074 Aachen Germany Corresponding author Mark Coburn mcoburn@ Received 26 Jan 2009 Revisions requested 28 Feb 2009 Revisions received 18 Mar 2009 Accepted 27 Apr 2009 Published 27 Apr 2009 Critical Care 2009 13 R61 doi cc7795 This article is online at http content 13 2 R61 2009 Rossaint et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction The anaesthetic agent propofol 2 6-diisopropylphenol has been shown to be an effective neuroprotective agent in different in vitro models of brain injury induced by oxygen and glucose deprivation. We examined its neuroprotective properties in an in vitro model of traumatic brain injury. Methods In this controlled laboratory study organotypic hippocampal brain-slice cultures were gained from six- to eight-day-old mice pups. After 14 days in culture hippocampal brain slices were subjected to a focal mechanical trauma and subsequently treated with different molar concentrations of propofol under both normo- and hypothermic conditions. After 72 hours of incubation tissue injury assessment was performed using propidium iodide PI a staining agent that becomes fluorescent only when it enters damaged cells via perforated .
