Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Bench-to-bedside review: The promise of rapid infection diagnosis during sepsis using polymerase chain reaction-based pathogen detection. | Available online http content 13 4 217 Review Bench-to-bedside review The promise of rapid infection diagnosis during sepsis using polymerase chain reaction-based pathogen detection Paul M Dark1-3 Paul Dean4 and Geoffrey Warhurst2 3 5 intensive Care Unit Salford Royal NHS Foundation Trust Stott Lane Salford Greater Manchester M6 8HD UK 2Infection Injury and Inflammation Research Group Salford Royal NHS Foundation Trust Stott Lane Salford Greater Manchester M6 8HD UK 3School of Translational Medicine University of Manchester Stott Lane Salford Greater Manchester M6 8HD UK 4Department of Anaesthesia and Critical Care East Lancashire Hospitals NHS Trust Royal Blackburn Hospital Haslingden Road Blackburn BB2 3HH UK 5Biomedical Sciences Research Institute University of Salford The Crescent Salford Greater Manchester M5 4WT UK Corresponding author Paul M Dark Published 15 July 2009 This article is online at http content 13 4 217 2009 BioMed Central Ltd Critical Care 2009 13 217 doi cc7886 Abstract Early infection diagnosis as the cause of a patient s systemic inflammatory syndrome is an important facet of sepsis care bundles aimed at saving lives. Microbiological culture provides the main route for infection diagnosis but by its nature cannot provide time-critical results that can impact on early management. Consequently broad-spectrum and high-potency antibiotics are essential during the immediate management of suspected sepsis in critical care but are associated with the development of drugresistant organisms and superinfections. Established molecular laboratory techniques based on polymerase chain reaction PCR technology can detect pathogen DNA rapidly and have been developed for translation into a clinical diagnostic setting. In the setting of sepsis in critical care emerging commercial systems are now available for the analysis of whole blood within hours with the presumed aim of adoption into the current
