Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: "FEM-based oxygen consumption and cell viability models for avascular pancreatic islets | Theoretical Biology and Medical Modelling BioMed Central Research FEM-based oxygen consumption and cell viability models for avascular pancreatic islets Peter Buchwald Open Access Address Diabetes Research Institute and the Department of Molecular and Cellular Pharmacology University of Miami Miller School of Medicine Miami FL USA Email Peter Buchwald - pbuchwald@ Published 16 April 2009 Received 6 November 2008 Theoretical Biology and Medical Modelling 2009 6 5 doi 1742-4682-6-5 Accepted 16 April 2009 This article is available from http content 6 1 5 2009 Buchwald licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The function and viability of cultured transplanted or encapsulated pancreatic islets is often limited by hypoxia because these islets have lost their vasculature during the isolation process and have to rely on gradient-driven passive diffusion which cannot provide adequate oxygen transport. Pancreatic islets islets of Langerhans are particularly susceptible due to their relatively large size large metabolic demand and increased sensitivity to hypoxia. Here finite element method FEM based multiphysics models are explored to describe oxygen transport and cell viability in avascular islets both in static and in moving culture media. Methods Two- and three-dimensional models were built in COMSOL Multiphysics using the convection and diffusion as well as the incompressible Navier-Stokes fluid dynamics application modes. Oxygen consumption was assumed to follow Michaelis-Menten-type kinetics and to cease when local concentrations fell below a critical threshold in a dynamic model it was also allowed to increase with increasing glucose concentration. Results .
