Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Cerebral microcirculation is impaired during sepsis: an experimental study. | Taccone et al. Critical Care 2010 14 R140 http content 14 4 R140 c CRITICAL CARE RESEARCH Open Access Cerebral microcirculation is impaired during sepsis an experimental study 11 112 2 Fabio Silvio Taccone Fuhong Su Charalampos Pierrakos Xinrong He Syril James Olivier Dewitte Jean-Louis Vincent1 Daniel De Backer1 Abstract Introduction Pathophysiology of brain dysfunction due to sepsis remains poorly understood. Cerebral microcirculatory alterations may play a role however experimental data are scarce. This study sought to investigate whether the cerebral microcirculation is altered in a clinically relevant animal model of septic shock. Methods Fifteen anesthetized invasively monitored and mechanically ventilated female sheep were allocated to a sham procedure n 5 or sepsis n 10 in which peritonitis was induced by intra-abdominal injection of autologous faeces. Animals were observed until spontaneous death or for a maximum of 20 hours. In addition to global hemodynamic assessment the microcirculation of the cerebral cortex was evaluated using Sidestream DarkField SDF videomicroscopy at baseline 6 hours 12 hours and at shock onset. At least five images of 20 seconds each from separate areas were recorded at each time point and stored under a random number to be analyzed using a semi-quantitative method by an investigator blinded to time and condition. Results All septic animals developed a hyperdynamic state associated with organ dysfunction and ultimately septic shock. In the septic animals there was a progressive decrease in cerebral total perfused vessel density from at baseline to n mm at shock onset P functional capillary density from to n mm P the proportion of small perfused vessels from 95 3 to 85 8 P and the total number of perfused capillaries from to n mm P . There were no significant changes in microcirculatory flow index over time. In sham animals the cerebral .
