Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Interleukin-10 gene down-expression in circulating mononuclear cells during infusion of drotrecogin-a activated: a pilot study. | Lavaux et al. Critical Care 2010 14 R163 http content 14 5 R163 c CRITICAL CARE RESEARCH Open Access Interleukin-10 gene down-expression in circulating mononuclear cells during infusion of drotrecogin-a activated a pilot study 1 2 3 11 2 Thomas Lavaux Pascal Bilbault Anne Launoy Marie-Pierre Gaub Pierre Oudet Francis Schneider Abstract Introduction The purpose of this study was to investigate the gene expression of interferon-gamma IFN-g tumor necrosis factor-alpha TNF-a and interleukin-10 IL-10 in circulating mononuclear cells harvested from septic shock patients on drotrecogin-a activated DAA in order to determine whether this treatment has any effect on the inflammation phase. Methods We conducted a prospective cohort study in two intensive care departments. Blood samples were collected at inclusion T1 and 36 hours later T2 to measure plasma cytokines and the changes in intracellular TNF-a IL-10 and IFN-g mRNA expressions using the real-time quantitative polymerase chain reaction RT-qPCR . Thirty-two septic shock patients were included 16 with DAA at 24 pg kg h for 96 hours DAA and 16 control DAA- eligible but contraindicated for DAA because of low platelet count. Results The basal characteristics were similar in both groups mortality 50 plasma cytokine concentrations and baseline IFN-g TNF-a and IL-10 mRNA expressions DAA vs. DAA- . At T2 there was a significant IFN-g gene down-regulation in DAA but not in DAA- patients vs. P . In survivors DAA administration was associated with a down-expression of both IFN-g vs. P and IL-10 vs. P . In the non-survivors DAA infusion was associated with IL-10 over-expression when compared with survivors vs. P . Conclusions In this study lack of IL-10 gene down-expression despite a 36-hour infusion of DAA is an ominous sign in septic shock patients suggesting that