Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Open lung approach associated with highfrequency oscillatory or low tidal volume mechanical ventilation improves respiratory function and minimizes lung injury in healthy and injured rats. | Krebs et al. Critical Care 2010 14 R183 http content 14 5 R183 c CRITICAL CARE RESEARCH Open Access Open lung approach associated with high-frequency oscillatory or low tidal volume mechanical ventilation improves respiratory function and minimizes lung injury in healthy and injured rats 12 11 3 4 Joerg Krebs Paolo Pelosi Charalambos Tsagogiorgas Liesa Zoeller Patricia RM Rocco Benito Yard Thomas Luecke1 Abstract Introduction To test the hypothesis that open lung OL ventilatory strategies using high-frequency oscillatory ventilation HFOV or controlled mechanical ventilation CMV compared to CMV with lower positive end-expiratory pressure PEEP improve respiratory function while minimizing lung injury as well as systemic inflammation a prospective randomized study was performed at a university animal laboratory using three different lung conditions. Methods Seventy-eight adult male Wistar rats were randomly assigned to three groups 1 uninjured UI 2 saline washout SW and 3 intraperitoneal intravenous Escherichia coli lipopolysaccharide LPS -induced lung injury. Within each group animals were further randomized to 1 OL with HFOV 2 OL with CMV with best PEEP set according to the minimal static elastance of the respiratory system BP-CMV and 3 CMV with low PEEP LP-CMV . They were then ventilated for 6 hours. HFOV was set with mean airway pressure PmeanHFOV at 2 cm H2O above the mean airway pressure recorded at BP-CMV PmeanBP-CMV following a recruitment manoeuvre. Six animals served as unventilated controls C . Gas-exchange respiratory system mechanics lung histology plasma cytokines as well as cytokines and types I and III procollagen PCI and PCIII mRNA expression in lung tissue were measured. Results We found that 1 in both SW and LPS HFOV and BP-CMV improved gas exchange and mechanics with lower lung injury compared to LP-CMV 2 in SW HFOV yielded better oxygenation than BP-CMV 3 in SW interleukin IL -6 mRNA expression was lower during BP-CMV and HFOV compared
