Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: PD-L1 blockade improves survival in experimental sepsis by inhibiting lymphocyte apoptosis and reversing monocyte dysfunction. | Zhang et al. Critical Care 2010 14 R220 http content 14 6 R220 KS CRITICAL CARE RESEARCH Open Access PD-L1 blockade improves survival in experimental sepsis by inhibiting lymphocyte apoptosis and reversing monocyte dysfunction Yan ZhangH Ying Zhou2t Jingsheng Lou3t Jinbao Li3 Lulong Bo3 Keming Zhu3 Xiaojian Wan3 Xiaoming Deng3 Zailong Cai 1 Abstract Introduction Lymphocyte apoptosis and monocyte dysfunction play a pivotal role in sepsis-induced immunosuppression. Programmed death-1 PD1 and its ligand programmed death ligand-1 PD-L1 exert inhibitory function by regulating the balance among T cell activation tolerance and immunopathology. PD-1 deficiency or blockade has been shown to improve survival in murine sepsis. However PD-L1 and PD-1 differ in their expression patterns and the role of PD-L1 in sepsis-induced immunosuppression is still unknown. Methods Sepsis was induced in adult C57BL 6 male mice via cecal ligation and puncture CLP . The expression of PD-1 and PD-L1 expression on peripheral T cells B cells and monocytes were measured 24 hours after CLP or sham surgery. Additionally the effects of anti-PD-L1 antibody on lymphocyte number apoptosis of spleen and thymus activities of caspase-8 and caspase-9 cytokine production bacterial clearance and survival were determined. Results Expression of PD-1 on T cells B cells and monocytes and PD-L1 on B cells and monocytes were up-regulated in septic animals compared to sham-operated controls. PD-L1 blockade significantly improved survival of CLP mice. Anti-PD-L1 antibody administration prevented sepsis-induced depletion of lymphocytes increased tumor necrosis factor TNF -a and interleukin IL -6 production decreased IL-10 production and enhanced bacterial clearance. Conclusions PD-L1 blockade exerts a protective effect on sepsis at least partly by inhibiting lymphocyte apoptosis and reversing monocyte dysfunction. Anti-PD-L1 antibody administration may be a promising therapeutic strategy for .
