Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Hydrogen inhalation ameliorates ventilator-induced lung injury. | Huang et al. Critical Care 2010 14 R234 http content 14 6 R234 KS CRITICAL CARE RESEARCH Open Access Hydrogen inhalation ameliorates ventilator-induced lung injury 13 14 5 1 Chien-Sheng Huang Tomohiro Kawamura Sungsoo Lee Naobumi Tochigi Norihisa Shigemura D 4- -1 ft 1 D I r I 76 I A h 141 Z S l - 7 I i z D D I I I I K8 ri r A l í- I rr 3 1 A 4 r I I z VI l I 1 - Z S. 1 3 8 Bettina M Buchholz John D Kloke Timothy R Billiar Yoshiya loyoda Atsunori Nakao Abstract Introduction Mechanical ventilation MV can provoke oxidative stress and an inflammatory response and subsequently cause ventilator-induced lung injury VILI a major cause of mortality and morbidity of patients in the intensive care unit. Inhaled hydrogen can act as an antioxidant and may be useful as a novel therapeutic gas. We hypothesized that owing to its antioxidant and anti-inflammatory properties inhaled hydrogen therapy could ameliorate VILI. Methods VILI was generated in male C57BL6 mice by performing a tracheostomy and placing the mice on a mechanical ventilator tidal volume of 30 ml kg without positive end-expiratory pressure FiO2 . The mice were randomly assigned to treatment groups and subjected to VILI with delivery of either 2 nitrogen or 2 hydrogen in air. Sham animals were given same gas treatments for two hours n 8 for each group . The effects of VILI induced by less invasive and longer exposure to MV tidal volume of 10 ml kg 5 hours FiO2 were also investigated n 6 for each group . Lung injury score wet dry ratio arterial oxygen tension oxidative injury and expression of pro-inflammatory mediators and apoptotic genes were assessed at the endpoint of two hours using the high-tidal volume protocol. Gas exchange and apoptosis were assessed at the endpoint of five hours using the low-tidal volume protocol. Results Ventilation 30 ml kg with 2 nitrogen in air for 2 hours resulted in deterioration of lung function increased lung edema and infiltration of inflammatory cells.
