Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Variants in the Toll-interacting protein gene are associated with susceptibility to sepsis in the Chinese Han population. | Song et al. Critical Care 2011 15 R12 http content 15 1 R12 cs CRITICAL CARE RESEARCH Open Access Variants in the Toll-interacting protein gene are associated with susceptibility to sepsis in the Chinese Han population Zhenju Song Jun Yin Chenling Yao Zhan Sun Mian Shao Yaping Zhang Zhengang Tao Peizhi Huang Chaoyang Tong Abstract Introduction Deregulated or excessive host immune responses contribute to the pathogenesis of sepsis. Toll-like receptor TLR signaling pathways and their negative regulators play a pivotal role in the modulation of host immune responses and the development of sepsis. The objective of this study was to investigate the association of variants in the TLR signaling pathway genes and their negative regulator genes with susceptibility to sepsis in the Chinese Han population. Methods Patients with severe sepsis n 378 and healthy control subjects n 390 were enrolled. Five genes namely TLR2 TLR4 TLR9 MyD88 and TOLLIP were investigated for their association with sepsis susceptibility by a tag single nucleotide polymorphism SNP strategy. Twelve tag SNPs were selected based on the data of Chinese Han in Beijing from the HapMap project and genotyped by direct sequencing. The mRNA expression levels of TOLLIP were determined using real-time quantitative Polymerase Chain Reaction PCR assays and concentrations of tumor necrosis factor alpha TNF-a and interleukin-6 IL-6 were measured by enzyme-linked immunosorbent assay ELISA . Results Our results showed that the minor C-allele of rs5743867 in TOLLIP was significantly associated with the decreased risk of sepsis Padj odds ratio OR adj 95 confidence interval CI to after adjustment for covariates in multiple logistic regression analysis. A 3-SNP haplotype block harboring the associated SNP rs5743867 also displayed strong association with omnibus test P value of . Haplotype GTC showed a protective role against sepsis Padj while haplotype GCT showed an increased
